بررسی رابطه پلیمورفیسم در جایگاه اتصال miR-214 در ژن EGFR با سرطان ریه

Background and Aim: Lung cancer is the most common cause of cancer-related deaths wordwide and non-small cell lung cancer (NSCLC), especially adenocarcinoma, is the most common type of lung cancer. Most cases of adenocarcinoma will occur due to KRAS mutations or EGFR mutations and amplifications. The polymorphism rs884225 which is associated with increased EGFR expression is located in the 3'UTR of the EGFR within the miR-214 binding site, and near the miR-27 and miR-128 binding sites. This study aims to investigate the association between rs884225 and lung cancer in Isfahan population. Methods: This case-control study was conducted on genomic DNA from the blood samples of 111 healthy subjects and 61 patients. After finishing the primer design, the genotypes were determined using the Tetra Primer ARMS-PCR technique. The statistical analysis was performed on the Power Marker software, and the SISA website. Results: The frequency of the alleles C / T at rs884225 was almost similar among the Isfahan population with and without lung cancer, and a significant association between this polymorphism and lung cancer wasn't observed (CC: OR=2.24 ,p>0.05).The Odd Ratio (OR) of this polymorphism in different kinds of lung cancer was also determined in the population that were not statistically significant (CC in NSCLC :OR=4.6, p>0.05). Conclusion: There is no significant association between rs884225 and lung cancer among the Isfahan population and it doesn't seem to be a biological risk factor. Further studies are needed to reach more definite results, though.